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Multiplex PCR Protocols and Methods
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Multiplex PCR Protocols and Methods
Multiplex PCR amplifies multiple DNA targets using more than one pair of primers in one reaction tube. Multiplex PCR has been successfully applied on genetics screening, genotyping, diagnositic analysis of infectious disease etc. It is also cost-saving for large scale PCR analysis. Quantitative multiplex PCR requires extensive multiplex PCR reaction optimization to determine the primer concentration, DNA template concentration, PCR cycling condition and PCR buffer components.
Multiplex PCR primer design and experiment design (cool online tool to help you design multiplex pcr assay)
How to perform multiplex PCR optimization?
Multiplex PCR technique papers:
Garcia-Garcia AB, Blesa S, Martinez-Hervas S, Mansego ML, Gonzalez-Albert V, Ascaso JF, Carmena R, Real JT, Chaves FJ. Semiquantitative multiplex PCR: a useful tool for large rearrangement screening and characterization. Hum Mutat. 2006 Aug;27(8):822-8.
Beigel R., A.N., Apaydin S.M., Fortnow L., and Kasif S. 2001. An Optimal Multiplex PCR Protocol for Closing Gaps in Whole Genomes (RECOMB 01).
Tettelin, H., Radune, D., Kasif, S., Khouri, H. & Salzberg, S.L. 1999. Optimized multiplex PCR: efficiently closing a whole-genome shotgun sequencing project. Genomics 62,500-7.
Wittwer CT, Herrmann MG, Gundry CN, Elenitoba-Johnson KS. 2001. Real-time multiplex PCR assays. Methods. 25(4):430-42.
Markoulatos P, Siafakas N, Katsorchis T, Moncany M. Multiplex PCR: rapid DNA cycling in a conventional thermal cycler. J Clin Lab Anal. 2003;17(4):108-12.
Last update 08-Mar-2007, Rating Good of 5 votes.
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Write your comment
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Could you please ggive your experince and comments;
1. Any experince for methylation multiplex PCR?
2. What is MP (synthetic component) from QIAGEN?
3. How to stablize the multiplex PCR to be reproducible?
Rating: n/a
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i am using this for the identification of E.Coli strains causing diarrhea.
Rating: Very Good
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really good website.
do you have a software for primer designing of multiplex pcr? how to go about it
iam doing a project on it.
Rating: Very Good
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hello, i want to do the multiplex PCR for PRRS and PCV-2 in the same time, can you help me, becausa i can't to see a good diagnostic.
can i do??
because PRRS need firts RT-PCR and after the nested PCR, ante PCV-2 need only PCR
Rating: Excellent!
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Dear sir
I would like to use BLG media for researching on microbiology. Would you like to give me the media composition? Thank you very much.
With best regards
Yours Sincerely
Chenxiang Wang
Rating: Good
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Related resource
PCR and multiplex PCR: guide and troubleshooting
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Related new technical papers
A Multiplex PCR Method for Rapid Identification of Brachionus Rotifers. Mar Biotechnol (NY). 2008 Jul 1;
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