Comparative Genomic Hybridization (CGH)

CGH is a molecular cytogenetic method of screening a tumor for genetic changes. The alterations are classified as DNA gains and losses and reveal a characteristic pattern that includes mutations at chromosomal and subchromosomal levels.

Laboratory of Automated Image Analysis, Institute of Pathology at
Humboldt-University of Berlin.

Content:

CGH Images
CGH Protocols
CGH Online Tumor Database
CGH Database (internal use only)
CGH Related Method (LOH)
CGH Publications
CGH Links
Color Figures of Publications

Protocol include:

Metaphase chromosome preparation
DNA preparation by cryostom tissue dissection
DNA labeling by nick translation
Hybridization
DNA detection
Image capture
Image analysis
DAPI chromosome identification

Last update 15-Jun-2006, Rating Good of 2 votes.