Peptide Nucleic Acids: Protocols and Applications

Peptide Nucleic Acids: Protocols and Applications

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Content

Chapter 1. An Introduction to PNA
Peter E. Nielsen and Michael Egholm


Chapter 2. PNA Synthesis by Boc Chemistry
Troels Koch


Chapter 3. Synthesis of PNA Oligomers by Fmoc Chemistry
Ralph Casale, Ivar S. Jensen, and Michael Egholm


Chapter 4. Thermodynamics of PNA Interactions
Tommi Ratilainen, Anders Holm¨¦n and Bengt Nord¨¦n


Chapter 5. Peptide Nucleic Acid Targeting of Double-Stranded DNA
Thomas Bentin, H. Jakob Larsen, and Peter E. Nielsen


Chapter 6. PNA Beacons and Fluoroprobes
Bhaskar Datta, Miaomiao Wang, and Bruce A. Armitage


Chapter 7. Vector Tagging Via PNA
Alex G. Rebuffat, Alessio G. Bernasconi and Brigitte M. Frey


Chapter 8. PCR Clamping
Henrik ?rum


Chapter 9. PNA Directed Genome Rare Cutting: New Developments
Maxim D. Frank-Kamenetskii and Vadim V. Demidov


Chapter 10. PNA Openers for Duplex DNA: Basic facts, Fine Tuning and Emerging Applications
Vadim V. Demidov


Chapter 11. Combined Use of PNA and DNA for Fluorescence In Situ Hybridization (FISH)
Kirsten Vang Nielsen, Sven M¨¹ller, Tim Svenstrup Poulsen, Susanne Gabs, Andreas Sch?nau


Chapter 12. Production of PNA-Arrays for Nucleic Acid Detection
Anette Jacob, Ole Brandt, Simone W¨¹rtz, Achim Stephan, Martina Schn?lzer and J?rg D. Hoheisel


Chapter 13. PNA Antisense in Eukaryotic Systems
David R. Corey


Chapter 14. Antisense Effects in Escherichia coli
Liam Good, Rikard Dryselius and Peter E. Nielsen


Appendix

Guidelines for Sequence Design of PNA Oligomers
Handling and Storage
Buffers and Ionic Strength
Quantification
Melting Experiments
Trouble Shooting
Nomenclature

Last update 15-Mar-2005, Rating Excellent! of 1 votes.