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The principles of MS quantitation



The principles of MS quantitation

From IonSource maintained by Andrew Guzzetta.

Table of Contents:

1.Introduction to MS Quantitation
*Total Ion Current (TIC)
* Selected Ion Monitoring (SIM)
* Selected Reaction Monitoring (SRM / MRM)

2.Development of an SRM Transition

3.The Internal Standard

4.PK/LC/MS/MS Reverse Phase Chromatography Method Development

5.Sample Preparation

6.Standards and Standard Curve Preparation
* Standard Curve Creation Procedure
*Example Standard Curve Dilution Scheme
*Running the Standard Curve
*Randomizing Standards and Samples
*Running System Suitability
*The "QCs"

7. Links to PK Resources

Last update 16-Aug-2005, Rating Very Good of 15 votes.


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