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Blue Native Gel Electrophoresis for Thylakoid Protein Complexes

Blue Native Gel Electrophoresis for Thylakoid Protein Complexes

Protocol from Dr.Kenneth Cline's lab. The original protocol is used for chloroplasts, chromoplasts, and etioplasts study.

Last update 05-Jul-2006, Rating Poor of 2 votes.

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By Koel Sen on 10-Apr-2007

After using the higher concentration of chlorophyll and extensive solubilization with detergent all the bands appeared. But still no bands are very distinct. What could be the modified method?
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By KOEL SEN on 05-Jul-2006

I have performed BN PAGE very seriously according to your protocol. but getting only two faint bands in first dimension gel run.
Rating: Poor

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