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DNA Cloning Protocol



DNA Cloning Protocol

DNA cloning protocols content:

Preparation of oligo solutions
PCR experiments
Digestion of insert DNA
Digestion and dephosphorylation of vector DNA
Ligation of DNA fragments with sticky ends
Ligation of DNA fragments with blunt ends
Preparation of chemically competent E. coli cells
Transformation of plasmid DNA to chemically competent E. coli cells

(EMBL Hamburg)

Last update 10-Sep-2005, Rating Fair of 5 votes.


Post your message in Cloning library construction and screening forum

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By Mia on 19-Apr-2008
Does anyone have a protocol of shotgun cloning of Lambda-DNA fragments??

It's my first big experiment and I need help!! :(((

thanks
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By SHEETAL DHUMAL on 27-Oct-2007
THANKS FOR PROVIDING VERY NEAT PROTOCOL IN EACH SECTION OF MOLECULAR BIOLOGY WITH ADDITIONAL FACTORS AND IMPORTANT TIPS


Rating: Good

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By mahdi on 11-Nov-2006
please i need the protocol of cloning in tandem

(Salmonella or E coli )

thank you

Rating: Good

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By Fethi on 29-Apr-2006
please i need the protocol of cloning in tandem

(Salmonella or E coli )

thank you
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By ruchita selot on 10-Sep-2005
thanx a lot for providing with some of the most generally ignored tips while exp..
Rating: Good

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