Ethanol Precipitation of DNA Cycle Sequencing Reactions in 384-well plates (archive)
Protocol from Roe's lab.
This clearly is an "old procedure revisited" but is extremely efficient because
1) the amount of resulting nested fragment set is at least 5 to 10 times more than obtained by Sephadex G-50 filtration and
2) the signal strength on the ABI 3700 is increased approximately 5 to 10 fold.
Last update 26-Nov-2004, Rating n/a of 0 votes.