Protocol for Enhancing PCR of Very Difficult Regions
|
Protocol for Enhancing PCR of Very Difficult Regions
DNA templates with high GC content and short repeats sometimes are very difficult for PCR amplification. This PCR protocol for overcoming difficult DNA G/C rich regions uses Polyoxyethylenesorbitan monolaurate and Igepal CA-630. (Ziyun Yao, Shaoping Lin, HongMin Wu and B.A. Roe in Roe's lab)
Last update 24-Aug-2006, Rating Very Good of 1 votes.
|
Write your comment
|
For GC rich region, I 've been using Roche GC( Cat No:12 140 306 001) rich amplification kit for over 7 years. They are very good performance. the largest fragment I amplified form cDNA is 3.5 kb. It was said up to 5kb. But I never try , I use roche long PCR kit for 5kb amplification.
Rating: Very Good
Reply
|
|
Related resource
PCR across a highly GC rich tandem repeat region
|
