Lambda protocol
|
Lambda protocol
Protocol from Rob Phillips Group.
The well-known lambda bacteriophage grows on E. coli bacteria. Here's what you need to know to work with it.
Preparation of plates with rich medium
Preparation of liquid medium
Titering lambda
TM buffer for storage
Generating different sizes of lambda with ZAP and puc19
Purifying lamB, the lambda receptor
Propagating and purifying lambda phage
Extracting lambda DNA and testing on a gel
Last update 31-Oct-2005, Rating Very Good of 4 votes.
|
Write your comment
|
bonjour, je travaille dans un laboratoire de biologie moleculaire, j'ai besoin de connaitre les réferences les plus utiles pour travailler sur les levures et bacteries.
concernant les restricitions, le séquancage, la PCr ...
merci d'avance pour votre aide
Rating: Good
Reply
|
|
|
hi , my self rohan, i m doin MSc -2 microbiology, i m working with bacteriophages but i havent general protocol for isolation of phages. i need it because it is important for my project.
Rating: Fair
Reply
|
|
|
i get good info. from this site .send me other info. about plasmid vectors
Rating: Excellent!
Reply
|
|
|
Hi. I'm a biology's student. I'm working with bacteriophages, but I' don't have a general protocol for isolation of phages. I need it because it's important for my career's project.
Thanks.
Rating: Very Good
Reply
|
|
Related resource
E.Coli strain database (searchable)
E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology)
E.coli recombinant protein proteolysis
need help for the transformation
Best vector for expressing recombinant antibody fragments?
Protocol for making competent E coli
Plasmid or phagemid vector(s) holding large insert
Protein expression in E.Coli
|
