Technique / Molecular Biology / DNA-protein interactions

DNaseI footprinting

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	By Naveen on 27-Sep-2008 Hi, Could i get the protocol for performing the DNA footprinting, it will be help full. Rating: Good Reply

	By Noorie on 29-Jun-2007 Dear Sir I am doing Primer extension Dnase1 Footprinting. I get clear ladder of DNA in the lanes without the cell extract. However, in the lanes, with extracts i just smeary lanes. I have tried with different extracts already. Could you please give some suggestion Rating: Very Good Reply

	By Noorie on 29-Jun-2007 Dear Sir I am doing Primer extension Dnase1 Footprinting. I get clear ladder of DNA in the lanes without the cell extract. However, in the lanes, with extracts i just smeary lanes. I have tried with different extracts already. Could you please give some suggestion Rating: Very Good Reply

	By Shashi Kant on 13-Nov-2005 Hello Sir, I'm getting some problems regarding the foot printing assay. I have used same amount of radiolebelled probe in each reaction mixture but I am not getting same band intensity in each lane. This is a DNA precipitation problems or DNase I digestion problem. Could you please help me out. regards shashi Rating: Very Good Reply