Glycosylation

A question regarding glycosylation of proteins and how that looks in a
western. I have two different antibodies raised against the same protein. When
I use these on a western, in one case I get a tight band while (antiboby 1) and
in the other I get a smear of smaller molecular weight bands (antibody 2). I
don't think it is due to degradation since the first antibody gives a clean
signal (protein sample was the same for both and the same wetsern). Could this
be glycosylation?
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Last update 15-Nov-2000, Rating Good of 0 votes.