Freezing Cells
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Freezing Cells
Does any of you have any experience in freezing (and storing) single
cells at -20 dgr.C ?
What happens if a cell suspension (with DMSO) goes trough a temperature
profile of -10 to -70 degr.C?
Do you know of any references
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Freezing
Cells
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Last update 08-Jan-2001, Rating Fair of 1 votes.
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do cells expand when frozen?
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I put them in culture media with 10% DMSO,-80
for over night then liquid nitrogen for unlimited time,viability is always 95 to 99%.
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To freeze cells, I put them in 90% heat inactivated calf serum and 10% DMSO.and directly at -20 for 2 hours, at -80 overnight and then in liquid nitrogen for unlimited time.
Like this I have a great cell viability.
Good luck
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There is a quite good reference about Asymptote Cool Guide to Cryopreservation. You can read it online at http://www.asymptote.co.uk/manual/effects.htm.
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Related resource
Cell freezing protocol for cells growing in log phase
Non-enzymatic Methods for Cell Harvesting
Aspects of Cryo Preservation
Cryopreservation Application Guide
Trypan blue staining of cell culture
Thawing freezing vial of cells
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