Mitochondrial DNA: Methods and Protocols (Methods in Molecular Biology, Vol 197)

Mitochondrial DNA: Methods and Protocols (Methods in Molecular Biology, Vol 197)
by Copeland, William C. (National Institute of Environmental Health Sciences, Research Triangle Park, NC).

Table of content

1. Animal Models for Mitochondrial Disease
Wallace, Douglas C.
pp. 003-054

2. Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases
Schon, Eric A.; Naini, Ali; Shanske, Sara
pp. 055-074

3. Screening for Aging-Dependent Point Mutations in mtDNA
Michikawa, Yuichi; Attardi, Giuseppe
pp. 075-092

4. Scanning Low-Frequency Point Mutants in the Mitochondrial Genome Using Constant Denaturant Capillary Electrophoresis
Zheng, Weiming; Marcelino, Luisa A.; Thilly, William G.
pp. 093-106

5. Identification of mtDNA Mutations in Human Cancer
Nomoto, Shuji; Sanchez-Cespedes, Montserrant; Sidransky, David
pp. 107-117

6. In Situ PCR of the Common Human mtDNA Deletion: Is It Related to Apoptosis?
Zullo, Steven J.
pp. 119-128

7. Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCycler?
Wong, Alice; Cortopassi, Gino
pp. 129-137

8. Yeast Nuclear Genes for mtDNA Maintenance
Foury, Fran?oise
pp. 139-149

9. Measuring mtDNA Mutation Rates in Saccharomyces cerevisiae Using the mtArg8 Assay
Strand, Micheline K.; Copeland, William C.
pp. 151-157

10. Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR
Santos, Janine H.; Mandavilli, Bhaskar S.; Van Houten, Bennett
pp. 159-176

11. Migration of mtDNA into the Nucleus
Thorsness, Mary K.; White, Karen H.; Thorsness, Peter E.
pp. 177-186

12. 2D Gel Electrophoresis of mtDNA
Lorimer, Heather E.
pp. 187-196

13. Purification of Mitochondria for Enzymes Involved in Nucleic Acid Transactions
Bogenhagen, Daniel F.
pp. 199-210

14. Purification of Mitochondrial Uracil-DNA Glycosylase Using Ugi-Sepharose Affinity Chromatography
Bennett, Samuel E.; Shroyer, Mary Jane; Sung, Jung-Suk; Mosbaugh, Dale W.
pp. 211-225

15. Characterization of Specialized mtDNA Glycosylases
Nyaga, Simon G.; Bohr, Vilhelm A.
pp. 227-244

16. Purification, Separation, and Identification of the Human mtDNA Polymerase With and Without Its Accessory Subunit
Longley, Matthew J.; Copeland, William C.
pp. 245-257

17. Assay of mtDNA Polymerase ¦Ã from Human Tissues
Naviaux, Robert K.
pp. 259-271

18. Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: DNA Polymerase ¦Ã
Farr, Carol L.; Kaguni, Laurie S.
pp. 273-283

19. Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: Mitochondrial Single-Stranded DNA-Binding Protein
Farr, Carol L.; Kaguni, Laurie S.
pp. 285-294

20. Expression, Purification, and In Vitro Assays of Mitochondrial Single-Stranded DNA-Binding Protein
Li, Kang; Williams, R. Sanders
pp. 295-302

21. Recombinant Yeast mtDNA Helicases: Purification and Functional Assays
Kuusk, Silja; Sedman, Tiina; Sedman, Juhan
pp. 303-316

22. Isolation of DNA Topoisomerases I and II Activities from Mitochondria of Mammalian Heart
Low, Robert L.
pp. 317-329

23. Endonuclease G Isolation and Assays
Low, Robert L.; Gerschenson, Mariana
pp. 331-349

24. Targeting DNA Repair Proteins to Mitochondria
Dobson, Allison W.; Kelley, Mark R.; Wilson, Glenn L.; LeDoux, Susan P.
pp. 351-362

25. In Organello Footprinting of mtDNA
Ghivizzani, Steven C.; Madsen, Cort S.; Ammini, Chandramohan V.; Hauswirth, William W.
pp. 363-375

26. Crosslinking of Proteins to mtDNA
Kaufman, Brett A.; Newman, Scott M.; Perlman, Philip S.; Butow, Ronald A.
pp. 377-389

27. Breeding and Genotyping of Tfam Conditional Knockout Mice
Ekstrand, Mats; Larsson, Nils-G?ran
pp. 391-400

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