biowww.net
Home Forums /Molecular /Cell /Genetics /Proteomics /Neuroscience /Immunology /Bioinformatics /Histology /Pharmacology /Jobs /Books /Bioproducts /Blog /Methods
Search biowww:

Technique / Molecular Biology / Cloning library construction and screening


Subcloning oligos into expression vectors



Subcloning oligos into expression vectors

I've been trying to make several of constructs with various promoter
elements placed in front of a luciferase reporter gene (in a PGL3-based
vector) in order to test them for inducibility in my system. Unfortunately,
... ...
Link to original post



Replies:



Last update 15-Nov-2000, Rating Good of 0 votes.


Write your comment


Your Name
Your Email
Your Comment
Your Rating
Related resource
Manual Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation by a Cleared Lysate Method Fo


Some vector sequence and map


Protocols for ET cloning


High throughput recombination cloning in E. coli


Genomic cloning troubleshooting


Typical Cloning Strategies and Tips


CLONING PROBLEMS


cDNA library screening with antibody