Troubleshooting DNA sequencing data
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Troubleshooting DNA sequencing data
A detailed DNA sequencing data interpretation and troubleshooting guide (Greenwood molecular biology facility, University of Hawaii)
If your DNA sequencing data have following problems, you should follow the troubleshooting guide:
- No recognizable sequence
- Noisy data throughout sequence, with low signal strength
- Noisy data throughout sequences, with good signal strength
- Noise up to or after a specific point in the sequence
- Poor mobility correction
- Early signal loss
- Excess dye peaks at the beginning of the sequence in dye terminator chemistries
- Broad, red peak between base 200 and 350
- Pull-up peaks/bleedthrough
- Stop peaks in dye primer chemistry
- Compressions
- Poor data following a long homopolymer region
Last update 28-May-2002, Rating Good of 2 votes.
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hi all, i am sequencing what i thing is the 5' UTR of a gene to find the promoter region. I need softwares that can perform contig assembly and general sequence analysis....Can anyone help.
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i'm looking for explaination about A260/A280 value for DNA purity which extremely high foe example 11.5
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I have been seeing a lot of shoulder C peaks which are read as extra C's in our sequence, could you tell me what this is due to, or how to avoid it?
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hi,I'm looking for the full sequence of the binary vector pGA643.Can someone please tell me how or where to find it?
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Can someone please tell me where I can find the full sequence of pBabe puro vector?
Thanks
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I am looking for the full sequence of the pBabe puro vector.
Thanks you
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hi,
could you tell me how to submit my question?
I am a new user.
thank you very much!
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hi,
could you tell me how to submit my question?
I am a new user.
thank you very much!
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hi,
by the way,I think maybe you used TT virus as plate instead of SEN,but you don't know about it .
or maybe this exited a contamination in your PCR sytem.
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i am doing nested PCR for SEN virus using primers for 5 utr region. after doing sequencing PCR for the same, and sequencing by automated sequencer, blast search is matching the sequence with TT virus instead of SEN virus. even the sequence of amplicon from positive control is matching with TT virus. kindly help me out.
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it is very usefull
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nice troubleshooting.
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