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His-tags and DNA-binding

His-tags and DNA-binding

Has anyone come across a situation where a 6XHis tag has affected the
DNA-binding of a DNA-binding protein? (the pH is 8 so unlikely to be +ve
charge). I know the folding could be affected. Just wondered if anyone
had seen such a phenomenon...

Last update 01-Jun-2006, Rating n/a of 1 votes.

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By Sébastien on 01-Jun-2006

I have when purififying a phage repressor. It was inactive with a C-terminal 7his tag and perfectly active with an N-terminal 6his tag (though with a high tenedency for aggregation !).
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