Best way of cell cycle separation
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Best way of cell cycle separation
What's the best way to separate cells based on cell cycle phase?
I read about something called "counterflow centrifugal elutriation", but have
no idea what it is. Can you actually separate cells based on cell cycle phase
using FACS or does it just detect. Basically, I want to have different pools
of cell enriched for each phase of the cell cycle.
Original source
FACScan can only detect, if you want to sort cells you have to have a cell
sorter. Alternatively cells can be separated according to their denisity in
a special centrifuge (G1 cells contain less DNA and are therefore less
dense.... than G2/M cells)
Original source
Last update 12-Jun-2002, Rating Good of 0 votes.
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I also want to perform cell sorting to separate cells from whole-culture. Cell sorting function in FACS is difficult to perform. Would you tell me how to soring different phase cells using cytometer? Thanks every reply me!
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I want some books for the piochimstry ,I hope from you send me :
1-the price .
2-the book name.
3-the transfer price.
thank you so mutch.
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it's cool!
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Related resource
G1/S cell synchnronization using double thymidine block
Cell cycle methods (method booklet)
Cell synchronization protocol
Cell-cycle independent internal control genes
Flow Cytometric Protocols for Cell Cycle Analysis
BrdU and PI staining for cell cycle analysis
G1/S Phase Block for Cell Synchronization
Metaphase Block for Cell Synchronization
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