General recommendation for PCR sequencing

The most important factor in obtaining high quality sequence data is that your PCR amplification must be clean, robust and specific. One of the best methods of improving PCR specificity and reliability is to use a hot start PCR. This prevents the PCR reaction from beginning until elevated temperatures are reached and prevents mispriming due to potential regions of close homology in the genomic DNA. Primer-
dimers due to regions of complementary base pairing in the amplification primers are also largely prevented from forming in a hot start PCR reaction. There are... ...

Last update 28-Nov-2000, Rating Good of 0 votes.