Dual Bait yeast two-hybrid system

Protocol for construction of dual baid yeast two-hybrid system (Erica Golemis lab at Fox chase cancer center).

Protocol content:

- Main flowchart of Dual Bait protocol

Constructing and Transforming a cI Bait Protein

- Choice of cI-fusion vectors
- List of all available plasmids for cI part of Dual Bait
- Cloning into two-hybrid vectors
- Maps and sequences of cI-fusion vectors
- Polylinkers of two-hybrid basic vectors

Constructing and Transforming a LexA Bait Protein

- List of all available plasmids for LexA part of Dual Bait
- Cloning into two-hybrid vectors
- Maps and sequences of LexA-fusion vectors
- Polylinkers of two-hybrid basic vectors

Archived page

Last update 24-Nov-2000, Rating Good of 0 votes.