Technique / Molecular Biology / PCR / High fidelity long PCR

Long PCR Protocol

Long PCR Protocol

The key to LA PCR is an enzyme, TaKaRa LA TaqTM. It is a thermostable DNA polymerase, which possesses 3กฏ to 5กฏ exonuclease activity, or proofreading activity. The efficiency drastically declines when incorrect bases are incorporated. The 3กฏ to 5กฏ exonuclease activity removes these misincorporated bases and makes the further reaction proceed smoothly. Therefore, the amplification of long DNA fragments can be achieved. (Alam lab, University of Hawaii at Manoa)

Last update 24-Aug-2006, Rating Very Good of 2 votes.

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