Detection of Alu by PCR DNA Fingerprinting
|
Detection of Alu by PCR DNA Fingerprinting
Detection of Alu by PCR-A Human DNA Fingerprinting Lab Protocol
1994 Cold Spring Harbor Laboratory
DNA Learning Center
In this experiment, polymerase chain reaction (PCR) is used to amplify a nucleotide sequence from chromosome 8 to look for an insertion of a short DNA sequence called Alu within the tissue plasminogen activator (TPA) gene. Although the DNA from different individuals is more alike ... ...
Last update 15-Feb-2001, Rating Good of 1 votes.
|
Write your comment
|
I am stydying pcr and DNA work in beginning school.
I want to know about composition of PCR mix buffer in alu gene fingerprinting .(volume and concentration) thank you for reading
Rating: Very Good
Reply
|
|
|
I am looking for knock out and knock in protocol(including: 1. constract the targeting vector. 2. electroporate tne ES cells. 3. drug selection of resistant cells. 4. southen blotting or by PCR screen. 5. inject the selected clones into blastocy sts. 6. implant them into foster mice. if anyone has this protocol, please help me out.
sincerely yours
simon
Rating: Good
Reply
|
|
|
I am runing dection of TPA-25 gene on my PCR 9700 perkine elmer, but I need the protocol and an example of data so that I cane interprite them. Please please help me.
Professor Ihsan mahasneh
Member, new York Academy of Sciences
Rating: Good
Reply
|
|
Related resource
DNA fingerprinting protocol
DNA fingerprinting protocol for cell lines
|
