Gene targeted mice production

A technical mini-review on how to generate gene targeting mice (University of Wisconsin Biotechnology Center).

Content:
1. construction of targeting vector containing regions of identity with the mouse chromosome (homology units or arms), a selectable marker (generally a cassette that confers neomycin (G418) resistance) and planned modifications that ablate or alter the expression of the targeted gene or region of chromosome.
2. introduction of the linearized targeting vector into mouse ES cells and selection and screening for those targeted ES clones that have integrated the planned modifications by homologous recombination.
3. microinjection of targeted ES cells into blastocysts to generate mice chimeric for the targeted ES cells and host blastocyst cells.

Last update 04-May-2007, Rating Very Good of 1 votes.