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RNA extraction / RNA isolation guidelines and protocols



RNA extraction / RNA isolation guidelines and protocols

RNA extraction from cells and tissues guideline:

- Avoid RNA degradation caused by RNase contamination.
- Increase RNA yield by thourough homogenizatio of tissue and cell samples.
- Increase RNA quality by proper precipitation of RNA and optimized RNA storage condition.

Reference:
RNA isolation basics (Ambion)
Guidelines for working with RNA (Ambion)

Selected online protocols:

RNA Extraction Protocol from Tissue (Thomas Whisenant, using RNeasy column for total RNA purification)

RNA extraction from cells and RNA electrophoresis (PDF, Microarray facility, The University of Adelaide)

RNA Extraction and Quality Control (Roswellpark cancer institute, using Agilent RNA Quality Check)

Last update 18-May-2002, Rating Fair of 4 votes.


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