Technique / Bioinformatics

RNA extraction / RNA isolation guidelines and protocols


RNA extraction / RNA isolation guidelines and protocols

RNA extraction from cells and tissues guideline:

- Avoid RNA degradation caused by RNase contamination.
- Increase RNA yield by thourough homogenizatio of tissue and cell samples.
- Increase RNA quality by proper precipitation of RNA and optimized RNA storage condition.

Reference:
RNA isolation basics (Ambion)
Guidelines for working with RNA (Ambion)

Selected online protocols:

RNA Extraction Protocol from Tissue (Thomas Whisenant, using RNeasy column for total RNA purification)

RNA extraction from cells and RNA electrophoresis (PDF, Microarray facility, The University of Adelaide)

RNA Extraction and Quality Control (Roswellpark cancer institute, using Agilent RNA Quality Check)

Last update 18-May-2002, Rating Fair of 4 votes.

Write your comment

By boukoum on 06-Jul-2007
SVP je veux un protocole d'extraction d'ARN total à partir du culot sanguin et si c'est possible un protocole d'extraction avec le Trizol
Merci
Rating: n/a

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By Isidore on 12-Apr-2007
suis étudiant en annéé de DEA microbiologie. j suis a la recherche d'un protocole simple pour l'extraction des rotavirus dans les selles et leur migration électrophoretique. merci
Rating: n/a

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By Joe on 22-Dec-2006
When doing total RNA extraction from cell culture, is it better to add RNA extraction lysis buffer directly on top of the culture dish or better to trypsinize and wash in cold PBS before adding RNA isolation lysis buffer? Which way is better to avoid RNA degradation?
Rating: Excellent!

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By SHIDHI&ASWATHY on 15-Sep-2006
WE ARE THE FINAL YEAR BIOINFORMATICS STUDENTS SEEKING JOBS IN THE SAME ARE .KINDLY INFORM ABOUT THE JOB OPPORTUNITIES IN INDIA AND ABROAB

THANKING YOU
SHIDHI P.R
ASWATHY A.R
Rating: Good

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By Godwin on 18-May-2002
keep off igbo man
Rating: Good

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