Technique / Molecular Biology / PCR / PCR primer design

PCR primer design resources and tools

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	By nageswararao on 23-Jul-2005 how to design a primer for pBR322 Rating: Good Reply

	By k_kraitongsook on 02-Mar-2003 agctgatataaatatattttgaaccctaactatatctatatctatatatctatataattatatctatctatctatctatctatctatctatctatctatctatatatgtctatatatattgttcattttaacaacagcaca Rating: Good Reply

By Nat Ramani on 06-Jun-2001 I want to design a primer for ompF DNA to be used in primer extension using either isolated RNA or in vitro transcription RNA from E.coli. To design a primer where do I statrt? from + 1 or further downstream. The transcript size is around 150b. The ompF downstream sequnce is published by Norioka et al. J.Biol.Chem. (1986) 261; 17113-17119. I would appreciate your help in this matter. Thanking you Nat Ramani Rating: Good Reply