ELISA assay introduction
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ELISA assay introduction
Introduction to ELISA assay including:
1. Sandwich ELISA Protocol
2. Competitive ELISA Protocol
3. ELISA Troubleshooting
This excellent ELISA procedure introduction is provided by Chemicon.
Enzyme-linked Immunosorbent Assays (ELISAs) combine the specificity of antibodies with the sensitivity of simple enzyme assays, by using antibodies or antigens coupled to an easily assayed enzyme that possesses a high turnover number. ELISAs can provide a useful measurement of antigen or antibody concentration.
Last update 04-Mar-2001, Rating Fair of 3 votes.
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This forum is great. All my doubts have been answered except for one. In sandwich ELISA, why do enzymes such as HRPO and chromogen/substrate solution added from right to left of the titre plate.? I hope to be given an answer and full explanation regarding to the answer of this question. Thank you
Overall good forum
Rating: Very Good
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My question is related to ELISA for polysaccharide vaccine.Can u suggest some of the blocking agents or treatments to evade non specificity in ELISA for polysaccharide vaccines?
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My question is related to ELISA for polysaccharide vaccine.Can u suggest some of the blocking agents or treatments to evade non specificity in ELISA for polysaccharide vaccines?
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We are developing a ELISA based HCV Kit, in which various blocking agents have been tried,but we are getting high background, ranging from 0.15-3.2.please suggest us ,how this can be resolved.
Till we have used, BSA, Chick albumin, PVP, Casein digest,Milk protein, Horse serum, Rabbit serum,etc
Rating: Good
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Are there any other blocking agents except BSA, casein,milk?
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My question is rather general in some respects. Would extremely stringent washing of the ELISA plates during the ELISA procedure result in breaking the bonds between the antibody-enzyme-substrate interaction?
As well, how would bubbles, left from washing with washing buffer, affect the addition and binding of the antibody/enzyme/substrate?
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