Non-enzymatic Methods for Cell Harvesting
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Non-enzymatic Methods for Cell Harvesting
Nalge Nunc International.
In some cases it is necessary to harvest cells culture vessels without using protein digesting enzymes, proteases, such as trypsin, collagenase and pronase. These proteases may modify cellular proteins or cell membranes, thus affecting some immunological or physiological assays. Non-enzymatic dissociation solutions with EDTA (ethylenediaminetetraacetic acid) and ... ...
URL: Non-enzymatic Methods for Cell Harvesting
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Last update 26-Nov-2000, Rating Good of 0 votes.
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HI
This URL: Non-enzymatic Methods for Cell Harvesting has changed to
http://www.nuncbrand.com/page.asp?ID=1229&lang=GB
Good luck
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I have a serious problem with my cell line. They have been contaminated several times. I dont know what is wrong with them. Iam not using antibiotic but the cells should be allright. I am not sure if some body can advice me how to reduce or to prevent the contamination.
Thank you
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How does prolonged (up to 30min at 25-37 celcius) 2mM EDTA/PBS treatment affect cells? Can it cause the cells to "shrink", eg. due to changes in the cell cytoskeleton?
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I'd like to know your opinion regarding harvesting cells (in my case primary trophoblast cultures and choriocarcinoma cell lines) with:
- Tryspin
- Trypsin + EDTA
- EDTA alone
I noted a decrease in expression of Calcium-dependant cell membarne Adhesion molecules with use EDTA. Can this be because of the calium chelating effect of EDTA ?
Thank u
Beest regards
Dr. AlNasiry Salwan
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Anyone routinely use this non-enzymatic methods for dissociation cells? Does the cell viability is really higher than enzymatic method?
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