Technique / Molecular Biology / Bacterial plasmid bacterial phages vectors

Transformation efficiency


Transformation efficiency (archive)

If you are getting low transformation efficiency with chemically competent cells:

There were impurities in the DNA. Remove phenol, protein, detergents, and ethanol, by ethanol precipitation or GlassMAX DNA Isolation Systems. There was excess DNA. Use no more that 1-10 µg of DNA in <5 pl volume/100 pl of cells. (Shanta Dube, Life Technologies, Inc.)

Last update 28-Jun-2002, Rating Fair of 2 votes.

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By amna shah on 06-Jan-2006
the transformation efficiency is suspiciously TOO HIGH... what could have gone wrong??
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By srinivas reddy on 21-Jun-2005
iam srinivas a post graduate in bioinformatics(annamalai university,tamilnadu) now iam in searchof job in bioinformatics,so let me vacancies in hyderabad
Rating: Good

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By rafia.razzaque on 28-Jun-2002
the ligation reaction is not working
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