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Southern analysis with genomic fragment



Southern analysis with genomic fragment (archive)

I have to use a large fragment of DNA from a gen clone as a probe for a
Southern blot. I know it contain some repititive elementss. Is there a
protocol for pre-annealing the labelled DNA fragemn to block the repeatst
prior to hybridisation? Many thanks in advance
John
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Assuming your genomic fragment is human, try checking this Cot-1
competetive hyb info from Gibco BRL:


http://www2.lifetech.com/catalog/techline/molecular_biology/Manuals_PPS/15279011.pdf


It should work with mouse Cot-1 and genomic DNA, too
--Todd
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Last update 16-Jun-2008, Rating n/a of 1 votes.


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By jaya on 16-Jun-2008
I would like to determine the plasmid copy number of the recombinant cells in MCB and WCB by southern blot analysis.Is this method is recommended or other tham Q-PCR is there any method recommended?
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