Technique / Molecular Biology / PCR / PCR primer design


Adding restriction sites to PCR primers



Adding restriction sites to PCR primers

I am designing primers and I want to add restriction enzyme sites to the
5'ends. To do this, do I just add the restriction site to the 5' end, or do
I need to add extra bases after the enzyme's site so the the enzyme will be
able to cleave it? ... ...

Last update 18-Jul-2005, Rating Good of 1 votes.

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