Technique / Molecular Biology / PCR / PCR primer design
Adding restriction sites to PCR primers
Adding restriction sites to PCR primers
I am designing primers and I want to add restriction enzyme sites to the
5'ends. To do this, do I just add the restriction site to the 5' end, or do
I need to add extra bases after the enzyme's site so the the enzyme will be
able to cleave it? ... ...
Last update 18-Jul-2005, Rating Good of 1 votes.
