Technique / Proteomics and protein biochemistry / Protein expression, protein extraction and protein purification


Protease inhibitors



Protease inhibitors

Summary: Protease inhibitors used in protein extraction step.

Content: Protease inhibitors essential for prevent protein extracts from proteolysis, dephosphorylation, and other protein degradation/modification related activities.

Commonly used protease inhibitors include:

- EDTA (chelated calcium and magnesium ions)
- Sodium orthovanadate (tyrosine protein phosphatases inhibitor)
- Sodium fluoride (serine/threonine protein phosphatases inhibitor)
- Aprotinin
- Leupeptin (lysosomal protease inhibitor)
- Pepstatin (aspartic proteases inhibitor)
- PMSF (protease inhibitor)

Some vendors also provide cocktails or tablets of protease inhibitors. (Sigma, Roche etc)

Last update 03-Nov-2008, Rating n/a of 0 votes.

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