Technique / Proteomics and protein biochemistry / Protein electrophoresis
Protein electrophoresis
Protein electrophoresis
Protein electrophoresis overview
1. SDS-PAGE electrophoresis: use to determine the molecular weight of proteins.
Measuring Molecular Weight with SDS-PAGE (LogMW versus the relative mobility)
2. Native PAGE electrophoresis: use to isolate biological active proteins for enzyme activity or other biological activity measurement. Native gel will not denature proteins or reduce sulfhydryl groups of protein complex.
3. Isoelectric focusing gel electrophoresis (IEF): use IEF electrophoresis to determine the isoelectric point (pI) of your protein.
Two IEF gels: carrier ampholytes gels and immobilized pH gradient gels (IPG gels).
4. Two-dimensional (2-D) gel electrophoresis: Use for proteomics separation of proteins.
Last update 26-Jan-2009, Rating Very Good of 1 votes.
Write your comment
|
By neda
on 26-Jan-2009
hello i need a protocol with details for two dimentional electrophoresis |
Please Login or Register to Post
Resolving marginally different proteins on SDS-PAGE gel electrophoresis
Heavy and light chains on Coomaasie gel
Protein electrophoresis buffer recipes and running condition
Carbonate-based blotting transfer solution recipie?
Buffer recipes for making SDS polyacrylamide gel
Related peer-reviewed papers
Advances in serum protein electrophoresis.
Adv Clin Chem. 2006;42:43-80 Authors: Bossuyt X
Outcomes of discretionary laboratory requesting of serum protein electrophoresis.
Ann Clin Biochem. 2006 Sep;43(Pt 5):372-4 Authors: Hughes M, Davidson DF, McColl M
Two-dimensional protein electrophoresis: from molecular pathway discovery to biomarker discovery in neurological disorders.
NeuroRx. 2006 Jul;3(3):327-35 Authors: Choe LH, Werner BG, Lee KH
Understanding and interpreting serum protein electrophoresis.
Am Fam Physician. 2005 Jan 1;71(1):105-12 Authors: O'Connell TX, Horita TJ, Kasravi B
The diagnostic utility of serum protein electrophoresis.
Vet Clin North Am Exot Anim Pract. 1999 Sep;2(3):651-62 Authors: Werner LL, Reavill DR