ELISpot Assay

ELISpot: Digital quantification of the enzyme-linked immunospot

Enzyme-linked immunospot (ELISPOT) is a sensitive technique for the detection of cytokines released by immune cells. The technique is well established and correlates closely with the enzyme-linked immunosorbent assay (ELISA) technique. (Vaquerano et al., Biotechniques. 1998 Nov;25(5):830-4, 836.) ELISPOT analysis is typically performed using ELISPOT readers.

Principle of ELISpot assay

ELISpot assay method animation

ELISpot assay protocol (Gause Lab, Microbiology Department, Uniformed Services Univercity of Health Sciences)

ELISpot assay protocol from BioLegend

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