Original paper: 'Touchdown' PCR to circumvent spurious priming during gene amplification

This is the original paper on touchdown PCR technique by Don et al on Nucleic Acids Res. 1991 July 25; 19(14): 4008.

A frequently encountered problem in PCR amplification of target gene sequences, especially from complex genomes, is the
appearance of spurious smaller bands in the product spectrum. (Paper downloadable as pdf file)

Application of touchdown PCR technique:

1: Ding W, Zou H, Dai J, Duan Z.
Combining restriction digestion and touchdown PCR permits detection of trace
isoforms of histamine H3 receptor.
Biotechniques. 2005 Dec;39(6):841-5.

2: Levano-Garcia J, Verjovski-Almeida S, da Silva AC.
Mapping transposon insertion sites by touchdown PCR and hybrid degenerate
primers.
Biotechniques. 2005 Feb;38(2):225-9.

3: Rithidech K, Dunn JJ.
Combining multiplex and touchdown PCR for microsatellite analysis.
Methods Mol Biol. 2003;226:295-300.

4: Fietto JL, DeMarco R, Verjovski-Almeida S.
Use of degenerate primers and touchdown PCR for construction of cDNA libraries.
Biotechniques. 2002 Jun;32(6):1404-8, 1410-1.

5: Roux KH.
Using mismatched primer-template pairs in touchdown PCR.
Biotechniques. 1994 May;16(5):812-4.

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