Technique / Proteomics and protein biochemistry / Western blot assay

Western blot stripping protocol


Western blot stripping protocol

Western blot stripping protocol:

Buffer needed:

stripping buffer:
2% SDS
100 mM beta-mercaptoethanol
50 mM Tris, pH 6.8

Procedure:
1. Heat stripping buffer to 50c in water bath.
2. Incubate blot with stripping buffer at 50c for 15-30 min with gentle shaking.
3. Rinse blot several times in TBS.
4. Blot is now ready for re-block and blot.

Last update 29-Aug-2006, Rating Good of 6 votes.

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