Troubleshooting Fluorescent Microscopy Staining Errors

The troubleshooting guide gives detailed description on problems encountered in immunfluorescent microscopy. ( Michael W. Davidson and The Florida State University, Molecular Expressions)

Photomicrography under fluorescence illumination conditions presents a unique set of circumstances posing special problems for the microscopist. Exposure times are often exceedingly long (in some instances running from many seconds into several minutes), the specimen's fluorescence may fade during exposure, and totally black backgrounds often inadvertently signal light meters to suggest overexposure.

Last update 06-May-2008, Rating n/a of 1 votes.