comparative genomic hybridization (CGH) protocol

CGH protocol with flowchart illustrations from Institute of Pathology, "Rudolf-Virchow-Haus", University Hospital Charité, Humboldt-University of Berlin.

Steps:

Metaphase chromosome preparation
DNA preparation by cryostom tissue dissection
DNA labeling by nick translation
Hybridization
DNA detection
Image capture
Image analysis
DAPI chromosome identification

Last update 24-Jun-2006, Rating n/a of 0 votes.