Single-Cell Electroporation Protocol

A single-cell electroporation protocol for recombinant DNA transferation developed in Hollis Cline's lab at Cold Spring Harbor Laboratory.

This technique utilizes electroporation in which a brief external high voltage pulse induces sufficient transmembrane potential to disrupt the electrostatic forces maintaining lipid bilayer structure, causing the temporarily formation of small pores in the cell membrane. DNA and other charged molecules are then electrophoretically transferred into the cell through these pores. Following pulse termination, pores reseal over 10s to 100s of ms.

Last update 17-Jun-2006, Rating n/a of 0 votes.