Transfection of primary CNS and PNS neurons by electroporation

A pdf original article on neuron electroporation (Cecilia Y. Martinez and Peter J. Hollenbeck, Department of Biological Sciences, Purdue University).

Content:
I. Introduction
II. Electroporation Theory and Principles
III. Electroporation Procedure for Chick Primary Neurons
IV. Rationale for Setting Electroporation Parameters
A. Cell Density
B. Voltage and Duration of Pulse
C. Free Ca2+
D. Temperature
E. Rest for Recovery
F. Buffer Composition
V. Conclusions

Last update 17-Jun-2006, Rating n/a of 0 votes.