How to express toxic proteins in bacteria

I am trying to express my favorite gene cloned in pET vector in
E. Coli BL21. The N-terminal truncated protein (missing 13AAs) can be
expressed to high level after 1mM IPTG induction for 2 to 4 hours.
However the full length protein can not be expressed at all. After the
2.5 ml overnight culture (some times this culture grew fine) was put
into 50 ml LB, the bacteria grew very very slow as compared with the truncated clone. I guess that ... ...

Last update 28-Nov-2000, Rating Very Good of 1 votes.