Patti Lab Taqman Real time PCR protocol

Real-time quantitative multiplex RT -PCR (Taqman) protocol.

This is performed as a 2-step reaction:

1. cDNA synthesis from DNase I treated total RNA
2. multiplex real-time taqman PCR optimization and PCR.
This is a very detailed Taqman real time RT-PCR protocol from SC Patti Lab, Diabetes Genome Anatomy Project. (downloadable in pdf format)

Last update 20-Feb-2008, Rating Very Good of 1 votes.