Genotyping mice by real-time qPCR

Real-time PCR prototocol for genotyping Ts65Dn mice (Don Liu, Cecilia Schmidt, Tim Billings, and Muriel T. Davisson, The Jackson Laboratory).

Genotyping Ts65Dn mice is based on doing simultaneous quantitative PCR amplification of a gene or genes in the Ts65Dn chromosome and a control gene on another chromosome (in this case Apob) and comparing the average change (delta) in threshold cycle (CT) between the Ts65Dn genes and the control gene. Doing a multiplexed reaction with an internal control avoids the need for determining DNA concentration and permits a relatively large range of variation in concentration of template DNA.

Last update 02-Apr-2006, Rating n/a of 0 votes.