Adenovirus Production Protocol

A doc file of recombinant adenovirus production protocol. (Eric Kremer, Vect Euro Train)

Content:

I) Generation of the recombinantvector genome in a plasmid
II) Transfection of Recombinant Genome in transcomplementing cells
III) Preparation of an E1-deleted vector stock
IV) Purification of vector stock by banding on CsCl
V) Titration physical particles (by OD260) & PFUs (plaque forming units)
VIa) Titration of Infectious units
VIb) Infectious units assay by FACS

Last update 08-Feb-2006, Rating n/a of 0 votes.