Development of a Low-Cost, Colorometric Protein Array Systemto Measure Human Autoimmune Disease Markers

A free accessible original paper on protein array preparation.

Abstract:

A sensitive low-cost method for producing and visualizing protein microarrays was developed. This method utilizes a protein-specific microarray substrate that immobilizes proteins, therefore allowing for the detec-tion of protein-protein, protein-antibody, and protein-drug interactions. This protein array substrate, termed ¦Æ-grip, has been optimized to work with high sensitivity protein detection chemistries and with a nonfluorescent, low-cost, image-based detection system. Using this system, we validate the application of ¦Æ-grip in the analy-sis of autoimmune disease markers by screening patients diagnosed with autoimmune disorders, illustrating the feasibility, sensitivity, and repeatability of the above-mentioned substrate and system for this purpose.

Stewart J. Lebrun, Miragene, Inc., Anaheim, CA, USA

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