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Protocol for primary culture of human umbilical vein endothelial cells (HUVEC)

Protocol for primary culture of human umbilical vein endothelial cells (HUVEC)

A detailed protocol for primary cultures of HUVECs including buffer and reagent preparation, protocol and image. (PDF file, Professor Bruno Baudin)

Last update 20-Jul-2006, Rating Poor of 1 votes.

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By sheela.M.L. on 20-Jul-2006

I have been trying to isolate HUVEC's from the cord according to standard procedure followed by many scientist's. I am finding problem with bacterial contamination the next day after seeding the cells. It's also difficult to identify the cells.
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