Microarray protocols

Collections of microarray protocols, faqs and validation methods from Dr. Bart Frank, Arthritis and Immunology Program, OMRF.

Content:

Protocols
Reagent and Supply List
Detailed Slide Blocking Protocol
Direct Cy-Labeling Protocol with Omniscript RT (Modified Labeling Protocol for Ventana Discovery System)
Direct Cy-Labeling Protocol with EndoFree RT (Modified Labeling Protocol for Ventana Discovery System)
Indirect Cy-Labeling Protocol (OMRF Modified Amersham CyScribe Labeling Protocol)
Automated Hybridization Protocol (Ventana Discovery Optimized Protocol)

Frequently Asked Questions
How Much RNA Is Needed for Microarrays?
Which RNA Isolation Protocol Is Recommended?
Do you have other suggestions for working with RNA?
Do you print custom DNA libraries?
Validation
Quantitative Real-Time PCR (QRT PCR) Using Omniscript Reverse Transcriptase
Quantitative Real-Time PCR (QRT PCR) Using EndoFree Reverse Transcriptase

Last update 26-Oct-2005, Rating n/a of 0 votes.