RNAi Protocol Optimization Guide

A guidline for RNAi experiments design and protocol optimization. It is brought to you by BD bioscience with RNAi related products.

Content:

Getting Started
* I don’t know where to start
* I know which gene I’d like to “knockdown”. Now what?

Screening Your Knockdown Sequence
* I’d like to validate my shRNA sequence
* I need to screen a large number of potential knockdown sequences
* I’d like to use prevalidated knockdown sequences
* I’m not seeing good knockdown with the oligos or vector constructs that I’m using

Optimizing Gene Delivery
* I’ve achieved good knockdown with synthetic oligos. How do I get stable expression of my siRNA?
* I’m having difficulty transfecting my synthetic oligos or RNAi vector constructs
* I’m working with hard-to-transfect cell lines

Inducible Gene Silencing
* I’d like to use inducible gene silencing

Last update 25-Oct-2005, Rating n/a of 0 votes.