DNA cloning problem
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DNA cloning problem
We have been trying to clone a human gene in to TA vector. We've got
blue/white selection, have screened the white ones directly by PCR, and
then tried to grow up those with inserts - this is where the problem
starts. On trying to grow up for mini-preps, nothing happens. There is
absolutley no growth.
We have tried different media and both Kan and Amp selections (as well
as no antibiotics at all), but we just ... ...
Last update 19-Dec-2005, Rating n/a of 1 votes.
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We have been trying to clone a human gene in to TA vector. We've got
blue/white selection, have screened the white ones directly by PCR, and
then tried to grow up those with inserts - this is where the problem
starts. On trying to grow up for mini-preps, nothing happens. There is
absolutley no growth.
We have tried different media and both Kan and Amp selections (as well
as no antibiotics at all), but we just ... ...
Rating: n/a
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