SYBR Green Real-time PCR

A pdf powerpoint overview on SYBR green real-time PCR. It discussed different methods, data analysis using delta-CT etc (47 pages). (www.gene-quantification.info)

SYBR-green real-time is perhaps the best way to quantify gene expression in large scale with high sensitivity and accuracy if primers and PCR reactions are optimized. The method utilizes SYBR green I dye which can bind to double-strand DNA during PCR amplification and emit light upon excitation. The SYBR green PCR reaction requires properly designed primer pair, SYBR green PCR master mix available from several vendors, and DNA template. The major disadvantage is its non-specific binding to any double-stranded DNA, including primer dimers and non-specific products. Generally a dissociation curve stage is required after PCR amplification to make sure only one sharp peak is detected at the melting temperature of amplicon.

Last update 26-Jun-2005, Rating n/a of 0 votes.