Semi-quantitative PCR

I have to do semi-quantitative PCR in order to study the regulation of
expression of receptors mRNA. I've already done optimization of annealing
temp., MgCl2 conc., etc...
If I want to compare the level of two different mRNA in response of a
stimulation of the cells, which of the following methods is the right one ?

1. Search for the optimal cycle number of primers pairs for each receptor studied, stimulate the cells, isolate RNA and do the RT-PCR with optimal ... ...

Last update 18-Nov-2000, Rating Good of 0 votes.