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Hybridization buffer recipe

Hybridization buffer recipe

Does anybody out there know a successful recipe of hybridization
buffer without the denatured salmon sperm DNA for genomic southern
hybridization?
Thanks.
XY Zheng
Newsgroup archive

Sure... use your usual hyb buffer (5X SSC, 10X Denhardts, 0.05% Na
pyrophosphate) and just boost your SDS to above 1%. For southerns I
normally ue 2% and haven't needed salmon sperm for over 6 years...

The old genescreen manual clued me onto this...
Hope this helps,
David
Newsgroup archive

This works fine for me.

7% SDS
0.5M Sodium Phosphate pH 7.2
1mM EDTA
1% BSA

I had no problem. Good luck.
N Kaushik
Newsgroup archive

Last update 08-Nov-2000, Rating Good of 0 votes.

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